NY/T 908-2004
ActiveDiagnostic technique for caseous lymphadenitis in sheep and goat
羊干酪样淋巴结炎诊断技术
Catalogue
前言 → 1 范围 → 2 病原菌分离与鉴定 → 3 酶联免疫吸附试验 (ELISA) Foreword → 1 Scope → 2 Isolation and Identification of Pathogenic Bacteria → 3 Enzyme-Linked Immunosorbent Assay (ELISA)
Scope
This standard specifies two quarantine methods for caseous lymphadenitis in sheep and goat (abbreviated as CLA): isolation and identification of pathogenic bacteria and enzyme-linked immunosorbent assay. The isolation and identification of pathogenic bacteria specified in this standard are applicable to the diagnosis of caseous lymphadenitis in sheep and goat; the enzyme-linked immunosorbent assay is applicable to the diagnosis, quarantine, and epidemiological investigation of caseous lymphadenitis in sheep and goat.
本标准规定了羊干酪样淋巴结炎 (caseous lymphadenitis in sheep and goat, 简称CLA)病原菌分离与鉴定和酶联免疫吸附试验2种检疫方法。本标准规定的病原菌分离与鉴定适用于羊干酪样淋巴结炎的诊断，酶联免疫吸附试验适用于羊干酪样淋巴结炎的诊断、检疫及流行病学调查。
Keywords
Application Summary AI generated
Veterinary quarantine personnel and laboratory technicians use this standard to diagnose caseous lymphadenitis in sheep and goats. They confirm infection through pathogen isolation and identification or enzyme-linked immunosorbent assay. This standard is crucial for controlling the spread of the disease in flocks and conducting epidemiological investigations.
兽医检疫人员和实验室技术人员使用该标准来诊断羊干酪样淋巴结炎。他们通过病原菌分离鉴定或酶联免疫吸附试验来确认感染。该标准对于控制羊群中该疾病的传播和进行流行病学调查至关重要。
AI Summary AI generated
This standard specifies two diagnostic methods for caseous lymphadenitis in sheep and goats: pathogen isolation and identification, and enzyme-linked immunosorbent assay (ELISA). The isolation and identification method confirms Corynebacterium pseudotuberculosis through observation of clinical features, culture characteristics, colony morphology, Gram staining, and biochemical tests. The ELISA method uses enzyme-labeled rabbit anti-goat IgG for serological detection. This standard is applicable to diagnosis, quarantine, and epidemiological investigation of the disease, and is important for controlling flock health.
本标准规定了羊干酪样淋巴结炎的两种诊断方法：病原菌分离鉴定和酶联免疫吸附试验。病原菌分离鉴定通过观察发病特征、培养特性、菌落形态、革兰氏染色和生化试验来确认伪结核棒状杆菌。ELISA方法使用酶标兔抗羊IgG进行血清学检测。该标准适用于该病的诊断、检疫和流行病学调查，对控制羊群健康具有重要意义。
Key Sentences extracted from text
发病羊的淋巴结肿大，呈脓性干酪性坏死，病羊消瘦，生产性能下降，孕羊产出死胎，严重者死亡。
若在血清琼脂平板上出现细小的颗粒样，半透明，边缘不整齐，干燥，松脆的菌落，则为可疑菌落。
若见有革兰氏阳性，呈球形或细丝状一端或两端膨大呈棒状的细菌，排列不规则，常呈丛状或栅栏状，则为可疑伪结核棒状杆菌，需进一步做生化试验。
若葡萄糖、乳糖、麦芽糖、甘露糖发酵产酸不产气，而淀粉、蕈糖不发酵，则判定为伪结核棒状杆菌。
酶标兔抗羊IgG；购买酶标兔抗羊IgG，用时以稀释缓冲液作1:4000稀释。
Affected sheep have enlarged lymph nodes with purulent caseous necrosis, emaciation, decreased production performance, stillbirth in pregnant ewes, and death in severe cases.
If small granular, translucent, irregular-edged, dry, and friable colonies appear on serum agar plates, they are considered suspicious colonies.
If Gram-positive, spherical or filamentous bacteria with one or both ends swollen into a club shape are seen, arranged irregularly, often in clusters or palisades, they are suspected to be Corynebacterium pseudotuberculosis, requiring further biochemical tests.
If glucose, lactose, maltose, and mannose fermentation produce acid but no gas, while starch and trehalose are not fermented, it is identified as Corynebacterium pseudotuberculosis.
Enzyme-labeled rabbit anti-goat IgG: purchase enzyme-labeled rabbit anti-goat IgG, and dilute 1:4000 with dilution buffer before use.