NY/T 772-2004
AbolishedAvian Influenza Virus RT-PCR Test Method
禽流感病毒 RT-PCR 试验方法
Catalogue
前言 → 1 范围 → 2 规范性引用文件 → 3 实验室条件 → 4 试剂的准备 Foreword → 1 Scope → 2 Normative References → 3 Laboratory Conditions → 4 Reagent Preparation
Scope
This standard specifies the type-specific detection technology for avian influenza virus and the RT-PCR differential identification technology for avian influenza virus H5, H7 hemagglutinin subtypes and N1, N2 neuraminidase subtypes. This standard is applicable to the detection of avian influenza virus nucleic acid in avian tissues, secretions, excreta, and chicken embryo allantoic fluid.
本标准规定了禽流感病毒型特异性检测技术和禽流感病毒 H5、H7 血凝素亚型及 N1、N2 神经氨酸酶亚型的 RT-PCR 鉴别技术。本标准适用于检测禽组织、分泌物、排泄物和鸡胚尿囊液中禽流感病毒核酸。
Normative References
Keywords
Application Summary AI generated
Veterinary laboratories and animal quarantine agencies use this standard to detect avian influenza virus and its subtypes. It is used for rapid diagnosis of avian influenza infections, especially in avian tissues and secretions. This standard is important because it provides a standardized RT-PCR method, ensuring accuracy and comparability of test results.
兽医实验室和动物检疫机构使用该标准来检测禽流感病毒及其亚型。它用于快速诊断禽流感感染，特别是在禽类组织和分泌物中。该标准的重要性在于提供了标准化的RT-PCR方法，确保检测结果的准确性和可比性。
AI Summary AI generated
This standard specifies the RT-PCR detection method for avian influenza virus, covering the identification of H5, H7 hemagglutinin subtypes and N1, N2 neuraminidase subtypes. It is applicable for detecting viral nucleic acid in avian tissues, secretions, excreta, and chicken embryo allantoic fluid. The standard details laboratory zoning requirements, reagent preparation, and operational precautions, emphasizing contamination prevention and personal protection.
本标准规定了禽流感病毒RT-PCR检测方法，涵盖H5、H7血凝素亚型和N1、N2神经氨酸酶亚型的鉴别。适用于禽组织、分泌物、排泄物和鸡胚尿囊液中病毒核酸的检测。标准详细描述了实验室分区要求、试剂准备和操作注意事项，强调防止污染和个人防护。
Key Sentences extracted from text
本标准规定了禽流感病毒型特异性检测技术和禽流感病毒 H5、H7 血凝素亚型及 N1、N2 神经氨酸酶亚型的 RT-PCR 鉴别技术。
本标准适用于检测禽组织、分泌物、排泄物和鸡胚尿囊液中禽流感病毒核酸。
从事 RT-PCR 工作的实验室尽可能分区，根据条件划分出 RNA 提取区、基因扩增区、电泳区。
特别注意电泳后的琼脂糖凝胶要及时处理，避免对实验室造成污染。
注意个人防护和环境保护，电泳中用到的 EB 可诱发基因突变，试验中被 EB 污染的物品要有专用收集处，并通过焚烧进行无害化处理。
This standard specifies the type-specific detection technology for avian influenza virus and the RT-PCR differential identification technology for avian influenza virus H5, H7 hemagglutinin subtypes and N1, N2 neuraminidase subtypes.
This standard is applicable to the detection of avian influenza virus nucleic acid in avian tissues, secretions, excreta, and chicken embryo allantoic fluid.
Laboratories engaged in RT-PCR work should be partitioned as much as possible, dividing into RNA extraction area, gene amplification area, and electrophoresis area according to conditions.
Special attention should be paid to timely disposal of agarose gel after electrophoresis to avoid contamination of the laboratory.
Pay attention to personal protection and environmental protection; EB used in electrophoresis can induce gene mutations, and items contaminated with EB in the test should have a dedicated collection area and be harmlessly treated through incineration.