NY/T 680-2003
AbolishedEnzyme-linked immunosorbent assay for avian leukosis virus p27 antigen
禽白血病病毒p27抗原酶联免疫吸附试验方法
Catalogue
前言 → 1 范围 → 2 禽白血病病毒p27抗原酶联免疫吸附试验 → 附录A Foreword → 1 Scope → 2 Enzyme-linked immunosorbent assay for avian leukosis virus p27 antigen → Annex A
Scope
This standard specifies the enzyme-linked immunosorbent assay method for avian leukosis virus (ALV) p27 antigen. This standard is applicable to the detection of ALV p27 protein antigen in egg albumen and chicken embryo tissue cell culture.
本标准规定了禽白血病病毒(ALV)p27 抗原酶联免疫吸附试验方法。本标准适用于检测鸡蛋蛋清以及鸡胚组织细胞培养物中的 ALV p27 蛋白抗原。
Keywords
Application Summary AI generated
Veterinary laboratories and poultry farms use this standard to detect avian leukosis virus p27 antigen. It provides a standardized enzyme-linked immunosorbent assay method for screening egg albumen and chicken embryo tissue cell cultures, aiding in diagnosis and control of avian leukosis. This standard ensures consistent testing procedures, supporting poultry health management and disease prevention.
兽医实验室和禽类养殖企业使用该标准来检测禽白血病病毒p27抗原。通过酶联免疫吸附试验，可以快速筛查鸡蛋蛋清和鸡胚组织细胞培养物中的病毒抗原，用于禽白血病的诊断和防控。该标准为禽类健康监测和疾病控制提供了统一的技术方法，保障养殖业安全。
AI Summary AI generated
This standard specifies an enzyme-linked immunosorbent assay for detecting avian leukosis virus p27 antigen in egg albumen and chicken embryo tissue cell cultures. The method involves coating plates with anti-p27 antibody, blocking, sample addition, enzyme-conjugated antibody reaction, and color development. It provides a standardized protocol for laboratory diagnosis of avian leukosis, supporting disease surveillance and control in poultry.
本标准规定了禽白血病病毒p27抗原的酶联免疫吸附试验方法，适用于检测鸡蛋蛋清和鸡胚组织细胞培养物中的病毒抗原。方法包括使用抗p27抗体包被板、封闭、加样、酶标抗体反应和显色等步骤。该标准为禽白血病的实验室诊断提供了统一的技术规范，有助于疫病监测和防控。
Key Sentences extracted from text
本标准规定了禽白血病病毒(ALV)p27 抗原酶联免疫吸附试验方法。
本标准适用于检测鸡蛋蛋清以及鸡胚组织细胞培养物中的 ALV p27 蛋白抗原。
用包被液将抗 p27 蛋白抗体作 4000 倍稀释，每孔 100 μL 加入96孔聚苯乙烯板中。
每孔加 150 μL 质量浓度为 1% 的明胶封闭液，37℃反应60 min。
将待检样品加入其他各孔，每个待检样本加两孔，每孔 100 μL，37℃反应。
This standard specifies the enzyme-linked immunosorbent assay method for avian leukosis virus (ALV) p27 antigen.
This standard is applicable to the detection of ALV p27 protein antigen in egg albumen and chicken embryo tissue cell culture.
Dilute the anti-p27 protein antibody 4000-fold with coating buffer, add 100 μL per well to a 96-well polystyrene plate.
Add 150 μL of 1% gelatin blocking solution per well, incubate at 37°C for 60 min.
Add the test samples to the other wells, two wells per sample, 100 μL per well, incubate at 37°C.