NY/T 684-2003
AbolishedDiagnostic techniques for canine distemper
犬瘟热诊断技术
Catalogue
前言 → 1 范围 → 2 临床诊断要点 → 2.1 临床症状 → 2.2 病理变化 → 3 病毒分离与鉴定 → 3.1 材料准备 → 3.1.1 试剂 → 3.1.2 器材 Foreword → 1 Scope → 2 Key points of clinical diagnosis → 2.1 Clinical symptoms → 2.2 Pathological changes → 3 Virus isolation and identification → 3.1 Material preparation → 3.1.1 Reagents → 3.1.2 Equipment
Scope
This standard specifies diagnostic techniques for canine distemper (CD), including clinical diagnosis, isolation and identification of canine distemper virus (CDV), immunoenzyme test and immunoenzyme histochemistry methods. This standard is applicable to the diagnosis of canine distemper.
本标推规定了犬瘟热(CD)的临床诊断 ,犬瘟热病毒(CDV)的分离与鉴定 .免疫酶试验和免疫酶组织化学方法等诊断技术。本标谁适用于犬瘟热的诊断。
Keywords
Application Summary AI generated
Veterinary diagnostic laboratories and animal disease control agencies use this standard to confirm cases of canine distemper. It provides methods for clinical observation, virus isolation, and immunological testing to accurately determine whether dogs are infected with canine distemper virus. This standard establishes unified technical specifications for early diagnosis and epidemic control, playing a vital role in protecting canine health.
兽医诊断实验室和动物疫病防控机构使用本标准来确诊犬瘟热病例。通过临床观察、病毒分离和免疫学检测等方法，可以准确判断犬只是否感染犬瘟热病毒。该标准为犬瘟热的早期诊断和疫情控制提供了统一的技术规范，对保护犬类健康具有重要意义。
AI Summary AI generated
This standard specifies diagnostic techniques for canine distemper (CD), including clinical diagnosis, virus isolation and identification, immunoenzyme test, and immunoenzyme histochemistry. Clinical diagnosis covers symptoms (fever, respiratory signs, neurological signs) and pathological changes (thymic atrophy, multisystem inflammation, inclusion bodies). The virus isolation section details material preparation, reagents, and equipment. This standard is applicable to laboratory diagnosis and clinical confirmation of canine distemper, providing technical support for veterinary disease control.
本标准规定了犬瘟热（CD）的诊断技术，包括临床诊断、病毒分离与鉴定、免疫酶试验和免疫酶组织化学方法。临床诊断要点涵盖临床症状（如发热、呼吸道症状、神经症状）和病理变化（如胸腺萎缩、多系统炎症、包涵体形成）。病毒分离部分详细说明了材料准备、试剂和器材要求。该标准适用于犬瘟热的实验室诊断和临床确诊，为兽医防控提供技术依据。
Key Sentences extracted from text
本标推规定了犬瘟热(CD)的临床诊断 ,犬瘟热病毒(CDV)的分离与鉴定 .免疫酶试验和免疫酶组织化学方法等诊断技术。
CD 的临床表现多种多样 ,与 CDV 的毒力 ,环境条件 ,宿主的年龄 ,品种及免疫状态有关。
病犬体温升高至 40%以上 ,鼻流清涕至脓性鼻汁 ,脓性眼屎 ,有咳嗽 .呼吸急促等肺炎症状 ,腹下可见米粒大丘疹。
CDV 为泛嗜性病毒,对上皮细胞有特殊的亲和力 ,因此病娈分布非常广泛。
CDV 的包涵体通常呈嗜酸性 ,位于胞浆内 ,直径1 Km~ 5 Am , 可在粘膜上皮细胞 .网状细胞 .白细胞 ,神经胶质细胞和神经元中发现。
This standard specifies diagnostic techniques for canine distemper (CD), including clinical diagnosis, isolation and identification of canine distemper virus (CDV), immunoenzyme test and immunoenzyme histochemistry methods.
The clinical manifestations of CD are diverse and are related to the virulence of CDV, environmental conditions, host age, breed, and immune status.
The body temperature of sick dogs rises above 40°C, with nasal discharge from clear to purulent, purulent eye discharge, cough, rapid breathing and other pneumonia symptoms, and millet-sized papules on the abdomen.
CDV is a pantropic virus with a special affinity for epithelial cells, so the pathological changes are very widespread.
CDV inclusion bodies are usually eosinophilic, located in the cytoplasm, 1 μm to 5 μm in diameter, and can be found in mucosal epithelial cells, reticular cells, leukocytes, glial cells, and neurons.