NY/T 679-2003
AbolishedEnzyme immunoassay for porcine reproductive and respiratory syndrome
猪繁殖与呼吸综合症免疫酶试验方法
Catalogue
前言 → 1 范围 → 2 免疫酶试验 → 2.1 材料准备 → 2.2 操作方法 → 2.3 结果判定 → 附录A Foreword → 1 Scope → 2 Enzyme immunoassay → 2.1 Material preparation → 2.2 Operation method → 2.3 Result determination → Appendix A
Scope
This standard specifies the enzyme immunoassay and enzyme immunohistochemistry methods for porcine reproductive and respiratory syndrome (PRRS). This standard is applicable to the detection of porcine reproductive and respiratory syndrome virus (PRRSV) antibodies in swine serum and PRRSV antigen in tissues.
本标准规定了猪繁殖与呼吸综合症 ( PRRS) 免疫酶试验和免疫酶组织化学方法 = 本标雄适用于检测猪血清申的猪繁殖与呼吸综合症病毒( PRRSV ) 抗体和组织中的 PRRSV 抗原
Keywords
Application Summary AI generated
Veterinary laboratories and diagnostic institutions use this standard to detect antibodies and antigens of porcine reproductive and respiratory syndrome virus. It provides a unified technical method for disease monitoring, diagnosis, and control in swine herds. This standard is crucial for maintaining the health of the pig industry and preventing the spread of the disease.
兽医实验室和诊断机构使用该标准来检测猪繁殖与呼吸综合症病毒抗体和抗原。该标准为猪场疫病监测、诊断和防控提供了统一的技术方法。它对于保障养猪业健康发展和动物疫病防控具有重要意义。
AI Summary AI generated
NY/T 679-2003 is a Chinese agricultural industry standard that specifies enzyme immunoassay and enzyme immunohistochemistry methods for porcine reproductive and respiratory syndrome (PRRS). The standard details material preparation (including reagents, equipment, and samples), operational procedures, and result interpretation. It is applicable for detecting PRRSV antibodies in swine serum and PRRSV antigen in tissues, providing a unified technical specification for PRRS diagnosis and surveillance.
NY/T 679-2003是中国农业行业标准，规定了猪繁殖与呼吸综合症（PRRS）的免疫酶试验和免疫酶组织化学方法。标准详细描述了材料准备（包括试剂、器材和样品）、操作步骤和结果判定方法。该标准适用于检测猪血清中的PRRSV抗体和组织中的PRRSV抗原，为PRRS的诊断和监测提供了统一的技术规范。
Key Sentences extracted from text
本标准规定了猪繁殖与呼吸综合症 ( PRRS) 免疫酶试验和免疫酶组织化学方法。
本标雄适用于检测猪血清申的猪繁殖与呼吸综合症病毒( PRRSV ) 抗体和组织中的 PRRSV 抗原。
抗原涂片的制备: PRRSV 接种于"长至70 '~80%单层的 Marc-145 细胞。
在阴性血清对照 .阳性l清对照成立的情况下:即阴怅血清与正常细胞和病诗'襞:苌泗胞反应均无色;阳性血清与正常细胞反应无色,与病毒感染细胞女应呈棕黄色至棕褐色.即荭柒果:否则应重试。
待检血清与正常细胞和病毒感染细胞反应均呈尤色 ,即可判为 PRRSI u !不~怅。
This standard specifies the enzyme immunoassay and enzyme immunohistochemistry methods for porcine reproductive and respiratory syndrome (PRRS).
This standard is applicable to the detection of porcine reproductive and respiratory syndrome virus (PRRSV) antibodies in swine serum and PRRSV antigen in tissues.
Preparation of antigen slides: PRRSV is inoculated into Marc-145 cells grown to 70-80% monolayer.
Under the condition that the negative serum control and positive serum control are valid: the negative serum shows no color reaction with both normal cells and virus-infected cells; the positive serum shows no color reaction with normal cells, but shows brown-yellow to brown color reaction with virus-infected cells; otherwise, the test should be repeated.
If the test serum shows no color reaction with both normal cells and virus-infected cells, it can be judged as PRRSV antibody negative.