NY/T 577-2002
AbolishedAgar gel immunodiffusion test for caprine arthritis/encephalitis
山羊关节炎/脑炎琼脂凝胶免疫扩散试验方法
Catalogue
前言 → 1 范围 → 2 琼脂凝胶免疫扩散试验 Foreword → 1 Scope → 2 Agar gel immunodiffusion test
Scope
This standard specifies the technical requirements for the agar gel immunodiffusion test for caprine arthritis/encephalitis. This standard is applicable to the diagnosis and quarantine of caprine arthritis/encephalitis.
本标准规定了山羊关节炎/脑炎琼脂凝胶免疫扩散试验的技术要求。本标准适用于山羊关节炎/脑炎的诊断和检疫。
Keywords
Application Summary AI generated
Veterinary laboratories and quarantine authorities use this standard to diagnose caprine arthritis/encephalitis. It provides a simple and specific agar gel immunodiffusion test method for detecting antibodies in goat serum. The standard aligns with international requirements and is important for animal quarantine in international trade.
兽医实验室和检疫部门使用该标准来诊断山羊关节炎/脑炎。该标准提供了一种简便、特异的琼脂凝胶免疫扩散试验方法，用于检测山羊血清中的抗体。该标准与国际标准一致，对国际贸易中的动物检疫具有重要意义。
AI Summary AI generated
This standard specifies the agar gel immunodiffusion (AGID) test method for caprine arthritis/encephalitis (CAE). It is used to detect CAEV antibodies in goat serum and is applicable for diagnosis and quarantine. The standard details material preparation, agar plate preparation, hole punching, sample loading, and result interpretation. The method is simple, specific, and consistent with international standards, serving as a designated diagnostic method in international trade.
本标准规定了山羊关节炎/脑炎（CAE）的琼脂凝胶免疫扩散（AGID）试验方法。该方法用于检测山羊血清中的CAEV抗体，适用于该病的诊断和检疫。标准详细描述了材料准备、琼脂板制备、打孔、加样和结果判定等操作步骤。该技术简便、特异，与国际标准一致，是国际贸易中指定的诊断方法。
Key Sentences extracted from text
本标准规定了山羊关节炎/脑炎琼脂凝胶免疫扩散试验的技术要求。
本标准适用于山羊关节炎/脑炎的诊断和检疫。
将琼脂板放于打孔模式圈上(见图1),用打孔器按图样打梅花型孔,孔径7 mm,孔距2.5 mm。
按图上中孔加入抗原,2,4,6孔滴加阳性血清,1,3,5孔滴加被检血清,加满样品后,置37℃温盒内。
于24h,48h及72h观察是否有沉淀线出现,终判时间为72h。
This standard specifies the technical requirements for the agar gel immunodiffusion test for caprine arthritis/encephalitis.
This standard is applicable to the diagnosis and quarantine of caprine arthritis/encephalitis.
Place the agar plate on the punching pattern (see Figure 1), use a puncher to punch plum-blossom-shaped holes according to the pattern, with a hole diameter of 7 mm and a hole spacing of 2.5 mm.
Add antigen to the center hole, add positive serum to holes 2, 4, and 6, add test serum to holes 1, 3, and 5, fill the samples, and place in a 37°C humid chamber.
Observe for precipitation lines at 24h, 48h, and 72h, with the final judgment at 72h.