NY/T 1244-2006

Veterinary laboratory technicians and clinical diagnosticians use this standard to confirm cases of contagious pustular dermatitis (orf) in sheep and goats. It provides standardized methods for virus isolation, electron microscopy, animal inoculation, and serological testing to differentiate this disease from similar conditions like sheep pox and foot-and-mouth disease. This standard is critical for the surveillance and control of this notifiable animal disease in China.

兽医实验室和临床诊断人员使用本标准来确诊接触传染性脓疱皮炎（羊口疮）。通过病毒分离、电镜观察、动物接种和免疫扩散等方法，可以准确区分该病与羊痘、口蹄疫等类似疾病。该标准对于我国第三类动物疫病的防控和监测具有重要意义。

This standard specifies laboratory diagnostic methods for contagious pustular dermatitis (orf), including virus isolation, electron microscopy, animal inoculation, and agar gel immunodiffusion. It is applicable for clinical diagnosis and laboratory identification of the disease. The standard details sample collection, processing, cell culture, and virus isolation procedures, and emphasizes differentiation from sheep pox and foot-and-mouth disease. It provides technical support for veterinary authorities in the prevention and control of this Category III animal disease.

本标准规定了接触传染性脓疱皮炎（羊口疮）的实验室诊断方法，包括病毒分离、电镜检查、动物接种和琼脂凝胶免疫扩散试验。适用于该病的临床诊断和实验室鉴定。标准详细描述了样品采集、处理、细胞培养及病毒分离步骤，并强调该病与羊痘、口蹄疫的鉴别诊断。该标准为兽医部门防控第三类动物疫病提供了技术依据。