GB/T 27859-2011
ActiveChemicals--Sediment-water chironomid toxicity test—Spiked sediment method
化学品 沉积物-水系统中摇蚊毒性试验 加标于沉积物法
Catalogue
前言 → 引言 → 1 范围 → 2 规范性引用文件 → 3 术语和定义 → 4 原理 → 5 受试物信息 → 6 参考物质 → 7 质量保证与质量控制 → 8 试验方法描述 → 9 试验的设计 → 10 试验步骤 → 11 数据和报告 → 附录A → 附录B → 附录C → 附录D Foreword → Introduction → 1 Scope → 2 Normative References → 3 Terms and Definitions → 4 Principle → 5 Information on the Test Substance → 6 Reference Substance → 7 Quality Assurance and Quality Control → 8 Description of the Test Method → 9 Test Design → 10 Test Procedure → 11 Data and Reporting → Annex A → Annex B → Annex C → Annex D
Scope
This standard specifies the test method for assessing the toxicity of chironomids in sediment-water systems using the spiked sediment method. This standard is applicable to evaluating the effects of long-term exposure of chemicals on larvae of the freshwater dipteran genus Chironomus sp. in sediment-water.
本标准规定了加标于沉积物法评估沉积物-水系统中摇蚊毒性的试验方法。本标准适用于评估化学品长期暴露对于处在沉积物-水中的淡水双翅目摇蚊属(Chironomus sp.)幼虫的影响。
Normative References
Keywords
Application Summary AI generated
Environmental toxicology laboratories and chemical safety assessment agencies use this standard to test the long-term toxicity of chemicals to aquatic benthic organisms (chironomid larvae). By simulating chemical exposure in sediment, it evaluates effects on chironomid emergence and development, providing critical data for environmental risk assessment of chemicals. This standard is important for protecting aquatic ecosystems and informing chemical management policies.
环境毒理学实验室和化学品安全评估机构使用该标准来测试化学品对水生底栖生物（摇蚊幼虫）的长期毒性。该标准通过模拟化学品在沉积物中的暴露场景，评估其对摇蚊羽化和发育的影响，为化学品环境风险评估提供关键数据。它对于保护水生生态系统和制定化学品管理政策具有重要意义。
AI Summary AI generated
This standard specifies a test method for assessing the toxicity of chemicals to chironomid larvae in sediment-water systems using the spiked sediment method. First-instar larvae are exposed to sediment-water systems containing different concentrations of the test substance, and toxicity is evaluated by measuring emergence rate and development rate. The standard details test design, exposure conditions, quality control, and data analysis methods. It is applicable for evaluating the effects of long-term chemical exposure on freshwater chironomid larvae, providing a basis for environmental risk assessment.
本标准规定了加标于沉积物法评估化学品对沉积物-水系统中摇蚊幼虫毒性的试验方法。方法将一龄摇蚊幼虫暴露于含不同浓度受试物的沉积物-水系统中，通过测量羽化率、发育速率等指标评估毒性效应。标准详细描述了试验设计、暴露条件、质量控制及数据分析方法，适用于评估化学品长期暴露对淡水摇蚊幼虫的影响，为环境风险评估提供依据。
Key Sentences extracted from text
本标准规定了加标于沉积物法评估沉积物-水系统中摇蚊毒性的试验方法。
试验的最终结果为羽化的成虫总数和羽化时间。
为使试验有效，应满足下列条件：a)试验结束时，对照组的羽化率不应低于70%；b)对照容器中的C. riparius和C. yoshimatsui应在引入后的12 d～23 d内羽化为成虫；C. tentans则需要20 d～65 d。
将一龄摇蚊幼虫暴露于一系列含有不同浓度的受试物的沉积物-水系统中进行试验。
所测实验数据可用回归模型分析，以估计导致羽化率或幼虫存活率或生长率下降z%的浓度（如15%有效浓度EC15，半数有效浓度EC50等），或者用统计假设检验来测定无可观察效应浓度(NOEC)或者最低可观察效应浓度(LOEC)。
This standard specifies the test method for assessing the toxicity of chironomids in sediment-water systems using the spiked sediment method.
The final results of the test are the total number of emerged adults and the emergence time.
For the test to be valid, the following conditions shall be met: a) At the end of the test, the emergence rate in the control group shall not be less than 70%; b) C. riparius and C. yoshimatsui in the control containers shall emerge within 12 d to 23 d after introduction; C. tentans requires 20 d to 65 d.
First-instar chironomid larvae are exposed to a series of sediment-water systems containing different concentrations of the test substance.
The measured experimental data can be analyzed using regression models to estimate the concentration causing a z% reduction in emergence rate, larval survival, or growth (e.g., 15% effective concentration EC15, median effective concentration EC50), or using statistical hypothesis tests to determine the no observed effect concentration (NOEC) or the lowest observed effect concentration (LOEC).