GB/T 27857-2011
ActiveChemicals -- Anaerobic biodegradability of organic compounds in digested sludge -- By measurement of gas production
化学品 有机物在消化污泥中的厌氧生物降解性气体产量测定法
Catalogue
前言 → 引言 → 1 范围 → 2 规范性引用文件 → 3 受试物信息 → 4 方法概述 → 5 试验准备 → 6 试验程序 → 7 数据处理 → 8 质量保证与质量控制 → 9 降解抑制 → 10 测试报告 Foreword → Introduction → 1 Scope → 2 Normative References → 3 Test substance information → 4 Method overview → 5 Test preparation → 6 Test procedure → 7 Data processing → 8 Quality assurance and quality control → 9 Degradation inhibition → 10 Test report
Scope
This standard specifies the test substance information, method overview, test preparation, test procedure, data processing, quality assurance and quality control, and test report for the method of measuring gas production for anaerobic biodegradability of organic compounds in digested sludge. This standard is applicable to chemical substances with known water solubility. When using the standard prepared with precise dosages provided in ISO 10634[1], it is also applicable to poorly water-soluble and non-water-soluble chemical substances. When this standard is used for volatile substances, specific operational steps must be taken to verify step by step, for example, determining the airtightness of the test system during the test.
本标准规定了化学品有机物在消化污泥中的厌氧生物降解性气体产量测定法的受试物信息、方法概述、试验准备、试验程序、数据处理、质量保证与质量控制与测试报告。本标准适用于已知水溶性的化学物质。如采用ISO 10634[1]中提供的精确剂量配制的标准时，还适用于难水溶性及非水溶性的化学物质。本标准用于挥发性物质时，需采取特定的操作步骤一步一步验证，例如确定试验过程中试验系统的气密性等。
Normative References
Keywords
Application Summary AI generated
Environmental testing agencies and chemical assessment laboratories use this standard to determine the anaerobic biodegradation potential of organic compounds. By measuring the gas production (methane and carbon dioxide) from microbial decomposition in digested sludge, they evaluate the fate of chemicals in anaerobic environments such as sewage sludge digesters. This standard is crucial for predicting the persistence and ecological risk of chemicals under anaerobic conditions, filling a gap in anaerobic biodegradability assessment methods.
环境检测机构和化学品评估实验室使用该标准来测定有机物在厌氧条件下的生物降解潜力。通过测量消化污泥中微生物分解有机物产生的气体（甲烷和二氧化碳）产量，评估化学品在厌氧环境（如污水处理厂消化反应器）中的归趋。该标准对于预测化学品在厌氧条件下的持久性和生态风险至关重要，填补了厌氧生物降解性评价方法的空白。
AI Summary AI generated
This standard specifies a method for measuring the anaerobic biodegradability of organic compounds in digested sludge by gas production. The method involves washing and diluting digested sludge with low inorganic carbon, then incubating it with the test substance in sealed containers at 35°C for 60 days. Biodegradation is assessed by measuring the pressure increase from methane and carbon dioxide in the headspace. It is applicable to water-soluble, poorly water-soluble, and non-water-soluble substances, with special verification for volatiles. The test includes blank, reference, and toxicity controls, and final inorganic carbon measurement. Biodegradation is calculated as the ratio of total carbon in gas and liquid to the test substance's carbon content; 75%-80% indicates complete degradation.
本标准规定了测定化学品在消化污泥中厌氧生物降解性的气体产量法。方法将低浓度无机碳的消化污泥清洗稀释后，与受试物在密闭容器中于35℃培养60天，通过测量顶部空间气体（甲烷和二氧化碳）压力增加来评估降解程度。适用于水溶性、难水溶性及非水溶性物质，挥发性物质需特殊验证。试验需设置空白、参比物和毒性对照，并测定最终无机碳含量。生物降解率通过气体碳和液相无机碳总量与受试物碳含量比值计算，达到75%-80%可视为完全降解。
Key Sentences extracted from text
将低浓度(<10 mg/L)无机碳(IC)的消化污泥清洗后，稀释约10倍，使其总固体浓度达到1 g/L～3 g/L后，与碳浓度为20mg/L～100mg/L的受试物一起置于密闭的容器中，于35℃±2℃温度下培养60 d。
试验过程中，测定由于产生CO2和CH4引起的试验容器顶部空间压力的增加值。
受试物生物降解的碳量(无机碳加甲烷碳)可通过净气体产量及液相中通过减去空白值的净无机碳量计算得到。
厌氧生物降解程度通过计算厌氧条件下总无机碳量和甲烷的总碳量之和与受试物的含碳量(测定或计算)比值计算而得。
如果生物气体的产量能够达到理论气体产生量的75%～80%，就可推断是完全厌氧生物降解。
After washing the digested sludge with low concentration (<10 mg/L) of inorganic carbon (IC), dilute it about 10 times to achieve a total solids concentration of 1 g/L to 3 g/L, then place it together with the test substance at a carbon concentration of 20 mg/L to 100 mg/L in a closed container, and incubate at 35°C ± 2°C for 60 days.
During the test, measure the increase in pressure in the headspace of the test container caused by the production of CO2 and CH4.
The amount of carbon biodegraded from the test substance (inorganic carbon plus methane carbon) can be calculated from the net gas production and the net inorganic carbon in the liquid phase after subtracting the blank value.
The degree of anaerobic biodegradation is calculated by the ratio of the sum of total inorganic carbon and total methane carbon under anaerobic conditions to the carbon content (measured or calculated) of the test substance.
If the biogas production reaches 75% to 80% of the theoretical gas production, it can be inferred as complete anaerobic biodegradation.