NY/T 910-2004
ActiveDetermination of robenidini hydrochloridum in feeds - high-performance liquid chromatography
饲料中盐酸氯苯胍的测定 高效液相色谱法
Catalogue
前言 → 1 范围 → 2 规范性引用文件 → 3 原理 → 4 试剂 Foreword → 1 Scope → 2 Normative References → 3 Principle → 4 Reagents
Scope
This standard specifies the method for the determination of robenidini hydrochloridum in feeds using high-performance liquid chromatography. This standard is applicable to the determination of robenidini hydrochloridum in compound feeds, concentrated feeds, and additive premixed feeds, with a minimum detection concentration of 2.5 μg/kg.
本标准规定了用高效液相色谱仪测定饲料中盐酸氯苯胍的方法。本标准适用于配合饲料、浓缩饲料及添加剂预混合饲料中盐酸氯苯胍的测定，最低检测浓度为2.5 μg/kg。
Normative References
Keywords
Application Summary AI generated
Feed manufacturers and quality inspection agencies use this standard to determine the content of robenidini hydrochloridum in feeds. It ensures compliance with drug additive limits, safeguarding animal feed safety. The standard provides accurate and reliable results via HPLC, which is critical for feed quality control.
饲料生产企业和质检机构使用该标准来检测饲料中盐酸氯苯胍的含量。该标准确保饲料中药物添加剂的合规性，保障动物食品安全。它通过高效液相色谱法提供准确可靠的检测结果，对控制饲料质量至关重要。
AI Summary AI generated
This standard specifies an HPLC method for determining robenidini hydrochloridum in feeds, applicable to compound feeds, concentrated feeds, and additive premixes, with a detection limit of 2.5 μg/kg. The method involves methanol extraction, evaporation, dissolution in acidic methanol, separation on a reversed-phase column, and UV detection at 352 nm. It references GB/T 6682 and GB/T 14699, providing detailed reagent and procedural requirements to ensure accuracy and reproducibility.
该标准规定了饲料中盐酸氯苯胍的高效液相色谱测定方法，适用于配合饲料、浓缩饲料及添加剂预混合饲料，最低检测浓度为2.5 μg/kg。方法原理为甲醇提取、浓缩蒸干、酸性甲醇溶解后经反相柱分离，紫外检测器在352 nm处测定。标准引用了GB/T 6682和GB/T 14699，对试剂、仪器和操作步骤有详细要求，确保检测结果的准确性和重复性。
Key Sentences extracted from text
本标准规定了用高效液相色谱仪测定饲料中盐酸氯苯胍的方法。
本标准适用于配合饲料、浓缩饲料及添加剂预混合饲料中盐酸氯苯胍的测定，最低检测浓度为2.5 μg/kg。
试料中的盐酸氯苯胍用甲醇提取。
提取液经浓缩蒸干，用酸性甲醇溶解后，在HPLC反相柱上分离，紫外检测器(UV检测器)352 nm处测定。
除非另有说明，本标准所用试剂为分析纯，水为蒸馏水，符合GB/T 6682三级水的规定。
This standard specifies the method for the determination of robenidini hydrochloridum in feeds using high-performance liquid chromatography.
This standard is applicable to the determination of robenidini hydrochloridum in compound feeds, concentrated feeds, and additive premixed feeds, with a minimum detection concentration of 2.5 μg/kg.
Robenidini hydrochloridum in the test sample is extracted with methanol.
The extract is concentrated and evaporated to dryness, dissolved in acidic methanol, then separated on an HPLC reversed-phase column and detected at 352 nm with a UV detector.
Unless otherwise specified, the reagents used in this standard are of analytical grade, and water is distilled water, complying with the requirements of grade 3 water in GB/T 6682.