NY/T 405-2000
AbolishedRules for virus detection of virus-free seed (seedling) garlic
脱毒大蒜种蒜(苗)病毒检测技术规程
Catalogue
前言 → 1 范围 → 2 引用标准 → 3 定义 → 4 检测对象 → 5 抽样 → 6 检测方法 → 7 质量要求 Foreword → 1 Scope → 2 Normative References → 3 Definitions → 4 Detection Objects → 5 Sampling → 6 Detection Methods → 7 Quality Requirements
Scope
This standard specifies the virus detection methods and operating procedures for virus-free seed (seedling) garlic. This standard is applicable to the virus detection of virus-free seed (seedling) garlic.
本标准规定了脱毒大蒜种蒜(苗)的病毒检测方法和操作规程。本标准适用于脱毒大蒜种蒜(苗)的病毒检测。
Normative References
Keywords
Application Summary AI generated
Agricultural quality inspection agencies, garlic seed producers, and farmers use this standard to test virus-free garlic seed (seedling) for specific viruses. It ensures seed quality through standardized sampling and DAS-ELISA methods, preventing virus spread and supporting healthy garlic production.
农业质检机构、大蒜种蒜生产企业和种植户使用本标准来检测脱毒大蒜种蒜(苗)是否携带特定病毒。它通过规范化的抽样和双抗体夹心酶联免疫吸附检测法，确保种蒜质量，防止病毒传播，从而保障大蒜产业的健康发展。
AI Summary AI generated
This standard specifies the technical procedures for virus detection of virus-free garlic seed (seedling), including detection objects (Onion yellow dwarf virus and Garlic common latent virus), sampling methods (tissue culture seedlings and field sampling), detection method (DAS-ELISA), and quality requirements (tolerance rates for virus-infected plants at different seed grades). It is applicable for quality inspection and management of virus-free garlic seed (seedling).
本标准规定了脱毒大蒜种蒜(苗)的病毒检测技术规程，包括检测对象（洋葱黄矮病毒和大蒜普通潜隐病毒）、抽样方法（组培苗和田间抽样）、检测方法（双抗体夹心酶联免疫吸附法）以及质量要求（不同级别种蒜的病毒病株允许率）。适用于脱毒大蒜种蒜(苗)的质量检验和管理。
Key Sentences extracted from text
本标准规定了脱毒大蒜种蒜(苗)的病毒检测方法和操作规程。
检测对象主要选择生产上发生分布范围广、危害性大的病毒。
脱毒核心材料:每株必须检测。
扩繁苗:随机抽取1%-2%的扩繁苗检测。
凡酶联免疫检测呈阳性反应的为带毒种蒜。
This standard specifies the virus detection methods and operating procedures for virus-free seed (seedling) garlic.
The detection objects mainly select viruses that are widely distributed and highly harmful in production.
Virus-free core material: each plant must be tested.
Propagation seedlings: randomly sample 1%-2% of propagation seedlings for testing.
Any sample that shows a positive reaction in the ELISA test is considered virus-infected seed garlic.