NY/T 401-2000
ActiveRules for virus detection of virus-free seed (seedling) potatoes
脱毒马铃薯种薯(苗)病毒检测技术规程
Catalogue
前言 → 1 范围 → 2 引用标准 → 3 定义 → 4 检测对象 → 5 抽样 → 6 检测方法 → 7 质量要求 → 附录A → 附录B → 附录C Foreword → 1 Scope → 2 Normative References → 3 Definitions → 4 Detection Targets → 5 Sampling → 6 Detection Methods → 7 Quality Requirements → Annex A → Annex B → Annex C
Scope
This standard specifies the virus detection methods and operating procedures for virus-free seed potatoes and tissue-cultured plantlets. This standard is applicable to the virus detection of virus-free seed potatoes and tissue-cultured plantlets.
本标准规定了脱毒马铃薯种薯、组培苗的病毒检测方法和操作规程。本标准适用于脱毒马铃薯种薯、组培苗的病毒检测。
Normative References
Keywords
Application Summary AI generated
Agricultural quality inspection agencies, seed potato producers, and plant quarantine departments use this standard to test virus-free seed potatoes and tissue-cultured plantlets for specific viruses. It ensures seed potato quality through standardized testing procedures, preventing virus spread and supporting the healthy development of the potato industry. This standard is crucial for maintaining market order and promoting virus-free technology.
农业质检机构、种薯生产企业、植物检疫部门使用该标准来检测脱毒马铃薯种薯和组培苗是否携带特定病毒。它通过规范化的检测流程确保种薯质量，防止病毒传播，保障马铃薯产业健康发展。该标准对维护种薯市场秩序、推广脱毒技术具有重要意义。
AI Summary AI generated
This standard specifies virus detection methods and operating procedures for virus-free seed potatoes and tissue-cultured plantlets. Detection targets include PVX, PVY, PVS, PLRV viruses and PSTVd viroid. Methods cover indicator plant test, double antibody sandwich ELISA, return electrophoresis, and RT-PCR. The standard also defines sampling methods, quality requirements, and positive judgment criteria, aiming to standardize detection and ensure seed potato quality.
本标准规定了脱毒马铃薯种薯和组培苗的病毒检测方法与操作规程，适用于相关检测工作。检测对象包括PVX、PVY、PVS、PLRV四种病毒和PSTVd类病毒。检测方法涵盖指示植物检测、双抗体夹心酶联免疫检测（ELISA）、往返电泳和RT-PCR。标准还规定了抽样方法、质量要求及阳性判断标准，旨在实现检测的规范化和标准化，保障种薯质量。
Key Sentences extracted from text
本标准规定了脱毒马铃薯种薯、组培苗的病毒检测方法和操作规程。
脱毒苗是指应用茎尖组织培养技术获得的再生试管苗，经检测确认不带马铃薯X病毒(PVX)、马铃薯Y病毒(PVY)、马铃薯S病毒(PVS)、马铃薯卷叶病毒(PLRV)等病毒和马铃薯纺锤块茎类病毒(PSTVd)，才确认是脱毒苗。
检测对象：马铃薯X病毒(Potato virus X,PVX)；马铃薯Y病毒(Potato virus Y,PVY)；马铃薯S病毒(Potato virus S,PVS)；马铃薯卷叶病毒(Potato leaf roll virus,PLRV)；马铃薯纺锤块茎类病毒(Potato spindle tuber viroid,PSTVd)。
凡指示植物检测、酶联免疫检测、往返电泳检测或RT-PCR检测呈阳性反应者为带毒种薯(苗)。
各级种薯病株病毒允许率应符合GB 18133-2000中第5章的规定。
This standard specifies the virus detection methods and operating procedures for virus-free seed potatoes and tissue-cultured plantlets.
Virus-free plantlets refer to regenerated test-tube plantlets obtained by meristem tip tissue culture technology, which are confirmed to be free of viruses such as Potato virus X (PVX), Potato virus Y (PVY), Potato virus S (PVS), Potato leaf roll virus (PLRV), and Potato spindle tuber viroid (PSTVd) after testing.
Detection targets: Potato virus X (PVX); Potato virus Y (PVY); Potato virus S (PVS); Potato leaf roll virus (PLRV); Potato spindle tuber viroid (PSTVd).
Any seed potato (seedling) that tests positive by indicator plant test, ELISA, return electrophoresis, or RT-PCR is considered virus-infected.
The allowable virus disease plant rates for each class of seed potatoes shall comply with the provisions of Chapter 5 of GB 18133-2000.