GB/T 41212-2021
ActiveNanotechnologies—5-(and 6)-Chloromethyl-2’,7’ Dichloro-dihydrofluorescein diacetate (CM-H2DCF-DA) assay for evaluating nanoparticle-induced intracellular reactive oxygen species (ROS) production in RAW 264.7 macrophage cell line
纳米技术 荧光素二乙酸酯法检测纳米颗粒诱导巨噬细胞产生的活性氧
Application Summary AI generated
This standard specifies a fluorescence-based method using CM-H2DCF-DA to measure reactive oxygen species (ROS) generated inside RAW 264.7 macrophage cells upon exposure to nanoparticles. It is applied in nanotoxicology and nanomaterial safety assessment to evaluate the oxidative stress potential of engineered nanoparticles. The protocol is used by research laboratories and regulatory testing facilities to standardize in vitro cytotoxicity screening for nanomaterials.
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