NY/T 761.3-2004
Method for Determination of Multiple Pesticide Residues in Vegetables and Fruits - Part 3: Method for Determination of Carbamate Pesticide Residues in Vegetables and Fruits
蔬菜和水果中有机磷、有机氯、拟除虫菊酯和氨基甲酸酯类农药多残留检测方法 第3部分 蔬菜和水果中氨基甲酸酯类农药多残留检验方法
Catalogue
前言 → 1 范围 → 2 原理 → 3 试剂与材料 → 4 仪器设备 → 5 测定步骤 Foreword → 1 Scope → 2 Principle → 3 Reagents and Materials → 4 Apparatus and Equipment → 5 Determination Procedure
Scope
This part specifies the liquid chromatography method for the determination of multiple residues of eight carbamate pesticides, including aldicarb sulfone, aldicarb sulfoxide, methomyl, 3-hydroxycarbofuran, aldicarb, carbofuran, carbaryl, and isoprocarb, in vegetables and fruits. This part is applicable to the determination of residues of the above eight pesticides in vegetables and fruits.
本部分规定了蔬菜和水果中涕灭威砜、涕灭威亚砜、灭多威、3-羟基呋喃丹、涕灭威、克百威、甲萘威、异丙威8种氨基甲酸酯类农药多残留液相色谱检测方法。本部分适用于蔬菜和水果中上述8种农药残留量的检测。
Keywords
Application Summary AI generated
Food safety testing laboratories and agricultural quality inspection agencies use this standard to determine residues of eight carbamate pesticides in vegetables and fruits. It employs liquid chromatography and solid-phase extraction to ensure accurate and reliable results, playing a critical role in ensuring agricultural product safety and protecting consumer health.
食品安全检测实验室和农业质检机构使用本标准来检测蔬菜和水果中8种氨基甲酸酯类农药的残留量。该标准通过液相色谱和固相萃取技术，确保检测结果的准确性和可靠性，对保障农产品安全和消费者健康具有重要意义。
AI Summary AI generated
This standard is Part 3 of the NY/T 761-2004 series, specifying a liquid chromatography method for the determination of multiple residues of eight carbamate pesticides (aldicarb sulfone, aldicarb sulfoxide, methomyl, 3-hydroxycarbofuran, aldicarb, carbofuran, carbaryl, and isoprocarb) in vegetables and fruits. The method involves acetonitrile extraction, solid-phase extraction column cleanup, and post-column derivatization with fluorescence detection, suitable for qualitative and quantitative analysis of these pesticide residues. The standard was proposed by the Ministry of Agriculture and is published for the first time.
本标准为NY/T 761-2004系列的第3部分，规定了蔬菜和水果中8种氨基甲酸酯类农药（涕灭威砜、涕灭威亚砜、灭多威、3-羟基呋喃丹、涕灭威、克百威、甲萘威、异丙威）多残留的液相色谱检测方法。方法采用乙腈提取、固相萃取柱净化、柱后衍生荧光检测，适用于蔬菜和水果中上述农药残留的定性和定量分析。标准由农业部提出，首次发布。
Key Sentences extracted from text
样品中氨基甲酸酯类农药用乙腈提取，提取液采用固相萃取技术分离、净化，经浓缩后，带荧光检测器和柱后衍生系统的高效液相色谱进行检测。
外标法定性、定量。
方法所用试剂，凡未指明规格者，均为分析纯；水为蒸馏水。
从100mL具塞量筒中准确吸取10.00mL乙腈相溶液，放入150mL烧杯中，将烧杯放在80℃水浴锅上加热，杯内缓缓通入氮气或空气流，将乙腈蒸发近干。
将氨基柱用4.0mL甲醇+二氯甲烷(1+99)预洗条件化，当溶剂液面到达柱吸附层表面时，立即加入样品溶液，用15mL离心管收集洗脱液。
The carbamate pesticides in the sample are extracted with acetonitrile, and the extract is separated and purified using solid-phase extraction technology, concentrated, and then detected by high-performance liquid chromatography with a fluorescence detector and post-column derivatization system.
External standard method is used for qualitative and quantitative analysis.
All reagents used in the method, unless otherwise specified, are of analytical grade; water is distilled water.
Accurately pipette 10.00 mL of the acetonitrile phase solution from a 100 mL graduated cylinder into a 150 mL beaker, heat the beaker in an 80°C water bath, and gently introduce nitrogen or air flow into the beaker to evaporate the acetonitrile to near dryness.
Precondition the amino column with 4.0 mL of methanol + dichloromethane (1+99). When the solvent level reaches the surface of the column packing, immediately add the sample solution and collect the eluate in a 15 mL centrifuge tube.