NY/T 447-2001
ActiveMethod for the determination of pesticide residues in leek
韭菜中甲胺磷等七种农药残留检测方法
Catalogue
前言 → 1 范围 → 2 原理 → 3 试剂 → 4 仪器和设备 → 5 提取和净化 → 6 测定 → 7 结果 → 8 色谱图 Foreword → 1 Scope → 2 Principle → 3 Reagents → 4 Apparatus and equipment → 5 Extraction and cleanup → 6 Determination → 7 Results → 8 Chromatogram
Scope
This standard specifies the method for the determination of residues of seven pesticides in leek. This standard is applicable to the residue analysis of methamidophos, phorate, monocrotophos, parathion, isofenphos-methyl, chlorpyrifos, and carbofuran in leek, with the minimum detectable concentrations (mg/kg) being: 0.01, 0.01, 0.03, 0.02, 0.01, 0.02, 0.04, respectively.
本标准规定了韭菜中7种农药的残留量测定方法。本标准适用于韭菜中甲胺磷、甲拌磷、久效磷、对硫磷、甲基异柳磷、毒死蝉、峡喃丹的残留分析，其最低检出浓度分别为(mg/kg);0. 01,0. 01,0. 03,0. 02,0. 01,0. 02,0.04,
Keywords
Application Summary AI generated
Agricultural testing laboratories and food safety authorities use this standard to detect residues of seven common pesticides in leek. It helps ensure leek products meet safety standards, protects consumer health, and provides scientific support for pesticide use management.
农业检测机构和食品安全监管部门使用本标准来检测韭菜中七种常见农药的残留量。它帮助确保韭菜产品符合安全标准，保护消费者健康，并为农药使用管理提供科学依据。
AI Summary AI generated
This standard specifies a gas chromatography method for the determination of residues of seven pesticides (methamidophos, phorate, monocrotophos, parathion, isofenphos-methyl, chlorpyrifos, and carbofuran) in leek. The sample is extracted with organic solvents, purified by column chromatography, and detected using a gas chromatograph with a nitrogen-phosphorus detector, with quantification by external standard method. The minimum detectable concentrations range from 0.01 to 0.04 mg/kg, making it suitable for residue analysis of these pesticides in leek.
本标准规定了韭菜中甲胺磷、甲拌磷、久效磷、对硫磷、甲基异柳磷、毒死蜱和呋喃丹七种农药残留的气相色谱检测方法。样品经有机溶剂提取、柱层析净化后，用气相色谱仪氮磷检测器检测，外标法定量。方法的最低检出浓度在0.01-0.04 mg/kg之间，适用于韭菜中这些农药的残留分析。
Key Sentences extracted from text
本标准规定了韭菜中7种农药的残留量测定方法。
样品中农药用有机溶剂提取，经柱层析净化去除干扰物，用气相色谱仪氮磷检测器检测，根据色谱峰的保留时间定性，外标法定量。
称取样品20.0g(精确至0.1 g),置于组织捣碎机中，加人log氯化钠，100 mL丙酮和50 -L二氯甲烷，高速匀浆1 min，静置10 min，取100 mL上清液于圆底烧瓶中，用旋转蒸发器在40℃水浴中减压浓缩至约1. 0 MI，用10 u r工_石油醚转移两次并继续浓缩至约1. 0 MI，待净化。
根据样液中农药的含量情况，选定相近的标准工作液，标准工作液和样液中农药的响应值均应在仪器的检测线性范围内。
农药残留量按式(1)计算
This standard specifies the method for the determination of residues of seven pesticides in leek.
Pesticides in the sample are extracted with organic solvents, purified by column chromatography to remove interferences, detected by gas chromatography with a nitrogen-phosphorus detector, qualitatively identified by retention time of chromatographic peaks, and quantified by external standard method.
Weigh 20.0 g of sample (accurate to 0.1 g), place in a high-speed tissue homogenizer, add 10 g sodium chloride, 100 mL acetone and 50 mL dichloromethane, homogenize at high speed for 1 min, let stand for 10 min, take 100 mL of supernatant into a round-bottom flask, concentrate under reduced pressure on a rotary evaporator in a 40°C water bath to about 1.0 mL, transfer twice with 10 mL petroleum ether and continue to concentrate to about 1.0 mL, ready for cleanup.
According to the pesticide content in the sample solution, select a suitable standard working solution; the response values of both the standard working solution and the sample solution should be within the linear range of the instrument.
The pesticide residue content is calculated according to formula (1).