NY/T 403-2000
AbolishedRules for virus detection of virus-free mother tree and seedling apples
脱毒苹果母本树及苗木病毒检测技术规程
Catalogue
前言 → 1 范围 → 2 引用标准 → 3 定义 → 4 检测对象 → 5 抽样 → 6 检测方法 → 7 质量要求 → 附录A Foreword → 1 Scope → 2 Normative References → 3 Definitions → 4 Detection Objects → 5 Sampling → 6 Detection Methods → 7 Quality Requirements → Appendix A
Scope
This standard specifies the virus detection methods and operating procedures for virus-free apple mother trees and seedlings. This standard is applicable to virus detection of virus-free apple mother trees (rootstock original species, scions), tissue culture seedlings, and virus-free seedlings.
本标准规定了脱毒苹果母本树及苗木的病毒检测方法和操作规程。本标准适用于脱毒苹果母本树(砧木原种、接穗)、组培苗及脱毒苗木的病毒检测。
Normative References
Keywords
Application Summary AI generated
Apple growers and nursery enterprises use this standard to detect viruses in virus-free apple mother trees and seedlings. It helps ensure seedling quality and prevent virus spread, thereby safeguarding the healthy development of the apple industry. By standardizing detection methods, it improves accuracy and operability.
苹果种植者和苗木繁育企业使用这个标准来检测脱毒苹果母本树和苗木是否携带病毒。它帮助确保苗木质量，防止病毒传播，从而保障苹果产业的健康发展。该标准通过规范检测方法，提高了检测的准确性和可操作性。
AI Summary AI generated
This standard specifies the technical procedures for virus detection of virus-free apple mother trees and seedlings, including detection objects (Apple chlorotic leaf spot virus, Apple stem pitting virus, Apple stem grooving virus), sampling methods (every mother tree tested, tissue culture seedlings and nursery seedlings sampled proportionally), detection methods (indicator plant method and A protein sandwich enzyme-linked immunosorbent assay), and quality requirements (allowable rate of virus-infected mother trees is 0, for virus-free seedlings ≤3.0%). Appendix A details the solution preparation, sample preparation, and operation steps of the A protein sandwich ELISA method.
本标准规定了脱毒苹果母本树及苗木的病毒检测技术规程，包括检测对象（苹果褪绿叶斑病毒、苹果茎痘病毒、苹果茎沟病毒）、抽样方法（母本树每株检测，组培苗和出圃苗木按比例抽样）、检测方法（指示植物检测法和A蛋白夹心酶联免疫吸附检测法）以及质量要求（母本树病毒病株允许率为0，脱毒苗木≤3.0%）。附录A详细描述了A蛋白夹心酶联免疫吸附检测法的溶液配制、样品制备和操作步骤。
Key Sentences extracted from text
本标准规定了脱毒苹果母本树及苗木的病毒检测方法和操作规程。
检测对象主要选择生产上发生分布范围广、危害性大的病毒。
凡酶联免疫检测或指示植物检测呈阳性反应的为带毒株。
母本树:病毒病株允许率为0。
脱毒苗木:病毒病株允许率≤3.0%。
This standard specifies the virus detection methods and operating procedures for virus-free apple mother trees and seedlings.
The detection objects mainly select viruses that are widely distributed and highly harmful in production.
Any positive reaction in enzyme-linked immunosorbent assay or indicator plant detection is considered a virus-infected plant.
Mother tree: the allowable rate of virus-infected plants is 0.
Virus-free seedlings: the allowable rate of virus-infected plants is ≤3.0%.