NY/T 402-2000
AbolishedRules for virus detection of virus-free seed (seedling) sweet potatoes
脱毒甘薯种薯(苗)病毒检测技术规程
Catalogue
前言 → 1 范围 → 2 定义 → 3 检测对象 → 4 抽样 → 5 检测方法 → 6 质量要求 → 附录A → 附录B Foreword → 1 Scope → 2 Definitions → 3 Detection targets → 4 Sampling → 5 Detection methods → 6 Quality requirements → Annex A → Annex B
Scope
This standard specifies the virus detection methods and operating procedures for virus-free seed (seedling) sweet potatoes. This standard is applicable to the virus detection of virus-free seed (seedling) sweet potatoes.
本标准规定了脱毒甘薯种薯(苗)的病毒检测方法和操作规程。本标准适用于脱毒甘薯种薯(苗)的病毒检测。
Keywords
Application Summary AI generated
Agricultural quality inspection agencies, sweet potato seed producers, and farmers use this standard to test virus-free seed potatoes and seedlings for specific viruses. It ensures seed quality through standardized sampling and detection methods, preventing virus spread and supporting healthy sweet potato industry development.
农业质检机构、甘薯种薯生产企业和种植户使用该标准来检测脱毒甘薯种薯和种苗是否携带特定病毒。它通过规范化的抽样和检测方法，确保种薯质量，防止病毒传播，保障甘薯产业健康发展。
AI Summary AI generated
This standard specifies the technical procedures for virus detection of virus-free sweet potato seed potatoes and seedlings, including detection targets (SPFMV and SPLV), sampling methods (tissue culture seedlings and field sampling), detection methods (Dot-ELISA and indicator plant method), and quality requirements (allowable rates of virus-infected plants for each grade). The standard aims to standardize detection procedures, ensure the quality of virus-free seed potatoes, and promote the popularization of sweet potato virus elimination technology.
该标准规定了脱毒甘薯种薯和种苗的病毒检测技术规程，包括检测对象（SPFMV和SPLV）、抽样方法（组培苗和田间抽样）、检测方法（斑点酶联免疫吸附法和指示植物法）以及质量要求（各级种薯病毒病株允许率）。标准旨在规范检测流程，确保脱毒种薯质量，促进甘薯脱毒技术推广。
Key Sentences extracted from text
本标准规定了脱毒甘薯种薯(苗)的病毒检测方法和操作规程。
脱毒苗应用茎尖组织培养技术获得的再生试管苗，经检测确认不带甘薯羽状斑驳病毒(SPFMV)和甘落潜隐病毒(SPLV)，才确认是脱毒苗。
原原种:病毒病株允许率是0。
凡斑点酶联免疫吸附检测或指示植物检测呈阳性者为带毒薯(苗)。
检测方法主要采用指示植物检测和斑点酶联免疫吸附检测方法。
This standard specifies the virus detection methods and operating procedures for virus-free seed (seedling) sweet potatoes.
Virus-free seedlings are regenerated test-tube plantlets obtained by shoot tip tissue culture technology, confirmed by testing to be free of Sweet potato feathery mottle virus (SPFMV) and Sweet potato latent virus (SPLV), and only then are they confirmed as virus-free seedlings.
Pre-elite: the allowable rate of virus-infected plants is 0.
Any sample that tests positive by Dot-ELISA or indicator plant detection is considered a virus-carrying potato (seedling).
The detection methods mainly adopt indicator plant detection and dot enzyme-linked immunosorbent assay.